O-244 Mitochondrial fission factor (MFF) is required for female fertility and oocyte development

Abstract Study question To explore the role of Mitochondrial fission factor (MFF) in female fertility and oocyte development using a Mff knockout mouse model. Summary answer deficiency influences mitochondrial dynamics oocytes at germinal vesicle stage (GV), results premature ovarian failure (POF) infertility. What is known already regulates quality control (MQC) by influencing dynamics. MQC has critical effects on POF. However, little about fertility. design, size, duration The (Mff-/-) mice were generated via CRISPR-Cas9 system. Adult Mff-/- compared to wildtype (WT) mice. Female 2-month old from each group (Mff-/- or WT, n = 5) used assess fecundity for 10 months. follicle (n 4 mice), number GV 5 mice) metaphase II (MII) determined. Participants/materials, setting, methods mated with adult WT males fecundity. Follicle was evaluated ovaries after fixation, paraffin embedding, sectioning, followed hematoxylin eosin staining. MFF DRP1 protein expression western blot. Oocyte mitochondria stained Mito-Tracker Red CMXRos. Main chance deficient had significantly lower levels (0.056 vs. 1, p < 0.01) than ovaries. infertile (0 39, 0.05). Mature (8-week-old) weight (0.0043 0.011, 0.05) WT. 2-month-old primordial (220 775, p< 0.05), primary (128 580, secondary (29 82, antral follicles (5 23, numbers (18 37, MII (2 16, 0.001) In addition, expressed less key regulator (0.54 contained elongated aggregated discrete evenly distributed ooplasm oocytes. Herein, we found that played concerted development. Limitations, reasons caution present observations may not be applicable humans. are derived global Mff. It which kind cell plays vital Further investigation needed regulatory mechanism regulation. Wider implications findings This study provides important insights into relationship between Trial registration